Production of laccase by repeated batch semi-solid fermentation using wheat straw as substrate and support for fungal growth.
Identifieur interne : 000224 ( Main/Exploration ); précédent : 000223; suivant : 000225Production of laccase by repeated batch semi-solid fermentation using wheat straw as substrate and support for fungal growth.
Auteurs : Antriksh Gupta [Inde] ; Asim Kumar Jana [Inde]Source :
- Bioprocess and biosystems engineering [ 1615-7605 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Laccase, Protéines fongiques.
- composition chimique : Triticum.
- croissance et développement : Reishi.
- Biomasse, Techniques de culture cellulaire en batch.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Fungal Proteins, Laccase.
- chemistry : Triticum.
- growth & development : Reishi.
- Batch Cell Culture Techniques, Biomass.
Abstract
Repeated batch semi-solid fermentation (sSF) process using wheat straw substrate and fungal growth of Ganoderma lucidum on solid substrate was studied for production of laccase. pH showed significant effect on laccase production. Highest laccase activity with pH controlled to 5.0 in batch sSF was 15257.2 ± 353.4 U L- 1 on 9th day. In repeated batch process at pH 5.0, insoluble biomass substrate and fungal growth were reused after liquid part of medium was replaced with glucose, ammonium phosphate (best nitrogen source) and combined glucose and ammonium phosphate solution separately. Refilled to 80% w v- 1 of initial soluble sugar of first batch resulted in highest laccase production with peak activity after 4 days from replacement. Production of enzyme increased from 15257.2 U L- 1 in first batch to cumulative 90164.4 U L- 1 in 29 days after six repeated batches, productivity increased from 1680.2 to 3110.3 U L- 1 day- 1 (∼ 1.9 times) due to reductions in inhibitory effects and time required for fungal growth. Utilization of wheat straw in repeated batch sSF was supported by composition analysis and morphological changes (scanning electron microscopy) of substrate. Economic production of laccase using agricultural residues in repeated batch sSF could be possible.
DOI: 10.1007/s00449-018-2053-6
PubMed: 30536123
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011, India. janaak@nitj.ac.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Dr. B. R. 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R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011</wicri:regionArea><wicri:noRegion>144011</wicri:noRegion></affiliation></author><author><name sortKey="Jana, Asim Kumar" sort="Jana, Asim Kumar" uniqKey="Jana A" first="Asim Kumar" last="Jana">Asim Kumar Jana</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011, India. janaak@nitj.ac.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011</wicri:regionArea><wicri:noRegion>144011</wicri:noRegion></affiliation></author></analytic><series><title level="j">Bioprocess and biosystems engineering</title><idno type="eISSN">1615-7605</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Batch Cell Culture Techniques (MeSH)</term><term>Biomass (MeSH)</term><term>Fungal Proteins (biosynthesis)</term><term>Laccase (biosynthesis)</term><term>Reishi (growth & development)</term><term>Triticum (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Laccase (biosynthèse)</term><term>Protéines fongiques (biosynthèse)</term><term>Reishi (croissance et développement)</term><term>Techniques de culture cellulaire en batch (MeSH)</term><term>Triticum (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Fungal Proteins</term><term>Laccase</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Laccase</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Reishi</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Reishi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Batch Cell Culture Techniques</term><term>Biomass</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Techniques de culture cellulaire en batch</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Repeated batch semi-solid fermentation (sSF) process using wheat straw substrate and fungal growth of Ganoderma lucidum on solid substrate was studied for production of laccase. pH showed significant effect on laccase production. Highest laccase activity with pH controlled to 5.0 in batch sSF was 15257.2 ± 353.4 U L<sup>- 1</sup> on 9th day. In repeated batch process at pH 5.0, insoluble biomass substrate and fungal growth were reused after liquid part of medium was replaced with glucose, ammonium phosphate (best nitrogen source) and combined glucose and ammonium phosphate solution separately. Refilled to 80% w v<sup>- 1</sup> of initial soluble sugar of first batch resulted in highest laccase production with peak activity after 4 days from replacement. Production of enzyme increased from 15257.2 U L<sup>- 1</sup> in first batch to cumulative 90164.4 U L<sup>- 1</sup> in 29 days after six repeated batches, productivity increased from 1680.2 to 3110.3 U L<sup>- 1</sup> day<sup>- 1</sup> (∼ 1.9 times) due to reductions in inhibitory effects and time required for fungal growth. Utilization of wheat straw in repeated batch sSF was supported by composition analysis and morphological changes (scanning electron microscopy) of substrate. Economic production of laccase using agricultural residues in repeated batch sSF could be possible.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30536123</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-7605</ISSN><JournalIssue CitedMedium="Internet"><Volume>42</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Bioprocess and biosystems engineering</Title><ISOAbbreviation>Bioprocess Biosyst Eng</ISOAbbreviation></Journal><ArticleTitle>Production of laccase by repeated batch semi-solid fermentation using wheat straw as substrate and support for fungal growth.</ArticleTitle><Pagination><MedlinePgn>499-512</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00449-018-2053-6</ELocationID><Abstract><AbstractText>Repeated batch semi-solid fermentation (sSF) process using wheat straw substrate and fungal growth of Ganoderma lucidum on solid substrate was studied for production of laccase. pH showed significant effect on laccase production. Highest laccase activity with pH controlled to 5.0 in batch sSF was 15257.2 ± 353.4 U L<sup>- 1</sup> on 9th day. In repeated batch process at pH 5.0, insoluble biomass substrate and fungal growth were reused after liquid part of medium was replaced with glucose, ammonium phosphate (best nitrogen source) and combined glucose and ammonium phosphate solution separately. Refilled to 80% w v<sup>- 1</sup> of initial soluble sugar of first batch resulted in highest laccase production with peak activity after 4 days from replacement. Production of enzyme increased from 15257.2 U L<sup>- 1</sup> in first batch to cumulative 90164.4 U L<sup>- 1</sup> in 29 days after six repeated batches, productivity increased from 1680.2 to 3110.3 U L<sup>- 1</sup> day<sup>- 1</sup> (∼ 1.9 times) due to reductions in inhibitory effects and time required for fungal growth. Utilization of wheat straw in repeated batch sSF was supported by composition analysis and morphological changes (scanning electron microscopy) of substrate. Economic production of laccase using agricultural residues in repeated batch sSF could be possible.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Antriksh</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jana</LastName><ForeName>Asim Kumar</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, G T Road Bye Pass, Jalandhar, 144011, India. janaak@nitj.ac.in.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Bioprocess Biosyst Eng</MedlineTA><NlmUniqueID>101088505</NlmUniqueID><ISSNLinking>1615-7591</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><MeshHeadingList><MeshHeading><DescriptorName UI="D061249" MajorTopicYN="N">Batch Cell Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020940" MajorTopicYN="N">Reishi</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Laccase production</Keyword><Keyword MajorTopicYN="N">Repeated batch process</Keyword><Keyword MajorTopicYN="N">Semi-solid fermentation</Keyword><Keyword MajorTopicYN="N">White rot fungi</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>7</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30536123</ArticleId><ArticleId IdType="doi">10.1007/s00449-018-2053-6</ArticleId><ArticleId IdType="pii">10.1007/s00449-018-2053-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Gupta, Antriksh" sort="Gupta, Antriksh" uniqKey="Gupta A" first="Antriksh" last="Gupta">Antriksh Gupta</name></noRegion><name sortKey="Jana, Asim Kumar" sort="Jana, Asim Kumar" uniqKey="Jana A" first="Asim Kumar" last="Jana">Asim Kumar Jana</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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